Phosphate acetyltransferase (PTA） (C008B-267460)

High purity recombinant phosphotransacetylase (Bacillus subtilis) for use in research, biochemical enzyme assays and analytical testing applications. Specificity: Catalyses the reaction: Coenzyme A + acetyl phosphate = acetyl-CoA + phosphateTemperature Optima: 25°CpH Optima: 7.4Application examples: Applications for the measurement of acetic acid in the food, fermentation, wine, beverage and dairy industries.PROPERTIES 1. ELECTROPHORETIC PURITY: - Single band on SDS-gel electrophoresis (MW ~ 36,953) - Single major band on isoelectric focusing (pI ~ 5.3) 2. PHYSICOCHEMICAL PROPERTIES: Recommended conditions of use are at pH 7.4 and up to 25°C. 3. STORAGE AND USE CONDITIONS/RECOMMENDATIONS: The enzyme is supplied as an ammonium sulphate suspension containing 0.5 mg/mL BSA and should be stored at 4°C. For assay, this enzyme should be diluted in 100 mM Tris.HCl buffer, pH 7.4 containing 1 mg/mL BSA. Swirl to mix the enzyme suspension immediately prior to use. 

Specifications & Purity: High purity recombinant phosphotransacetylase (Bacillus subtilis) for use in research, biochemical enzyme assays and analytical testing applications. Specificity: Catalyses the reaction: Coenzyme A + acetyl phosphate = acetyl-CoA + phosphateTemperature Optima: 25°CpH Optima: 7.4Application examples: Applications for the measurement of acetic acid in the food, fermentation, wine, beverage and dairy industries.PROPERTIES 1. ELECTROPHORETIC PURITY: - Single band on SDS-gel electrophoresis (MW ~ 36,953) - Single major band on isoelectric focusing (pI ~ 5.3) 2. PHYSICOCHEMICAL PROPERTIES: Recommended conditions of use are at pH 7.4 and up to 25°C. 3. STORAGE AND USE CONDITIONS/RECOMMENDATIONS: The enzyme is supplied as an ammonium sulphate suspension containing 0.5 mg/mL BSA and should be stored at 4°C. For assay, this enzyme should be diluted in 100 mM Tris.HCl buffer, pH 7.4 containing 1 mg/mL BSA. Swirl to mix the enzyme suspension immediately prior to use. 

Grade: Bioactive;Recombinant;ActiBioPure™;High Performance;EnzymoPure™

EC Number: 232-855-2

Storage Temperature: Store at 2-8°C

Shipped in: Wet ice

UPC:

51172415

Weight:

1.09 Ounces

HazmatClass:

Yes

Quantity:

1

Unit of Measurement:

EA

WeightUOM:

LB

MPN:

P1431341-250U

Country of Origin:

China

CAS:

9029-91-8

Product Size:

250U

Harmonized Tariff Code:

2931.90.90