Phospholipase D (PLD) Activity Assay Kit (Micro Method)

Phospholipase D (EC 3.1.4.4), also known as phosphatidylcholine hydrolase, is a general term for a class of enzymes that catalyze the hydrolysis of phosphodiester bonds and base exchange reactions. It is widely present in various organisms such as higher plants, animals, and bacteria, and performs physiological functions including involvement in cellular lipid metabolism, signal transduction, membrane formation, and stress resistance. Phospholipase D catalyzes the hydrolysis of the substrate O-(4-nitrophenyl)choline (NPPC). With the addition of exogenous acid phosphatase, this reaction produces p-nitrophenol (PNP), which has a maximum absorption peak at 405 nm. The PLD enzyme activity can be determined by measuring the increase rate of PNP at 405 nm.

Specifications & Purity: Phospholipase D (EC 3.1.4.4), also known as phosphatidylcholine hydrolase, is a general term for a class of enzymes that catalyze the hydrolysis of phosphodiester bonds and base exchange reactions. It is widely present in various organisms such as higher plants, animals, and bacteria, and performs physiological functions including involvement in cellular lipid metabolism, signal transduction, membrane formation, and stress resistance. Phospholipase D catalyzes the hydrolysis of the substrate O-(4-nitrophenyl)choline (NPPC). With the addition of exogenous acid phosphatase, this reaction produces p-nitrophenol (PNP), which has a maximum absorption peak at 405 nm. The PLD enzyme activity can be determined by measuring the increase rate of PNP at 405 nm.

Grade: BioReagent

Storage Temperature: Store at 2-8°C;Protected from light

Shipped in: Wet ice

UPC:

41141825

Weight:

1.09 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

ST

WeightUOM:

LB

MPN:

P1522103-48T

Country of Origin:

China

Product Size:

48T

Harmonized Tariff Code:

2931.90.90