PNV 35B ANTI-OSTEOCLASTOMA IGM

Cell Line Origin

Anti-Osteoclastoma Hybridoma

Cell Line Description

135B hybridoma was generated by fusing Ag876 myeloma cells with splenic cells of BALB/c mice immunised with tartrate-resistant acid phosphatase (TRAP) isolated from human osteoclastoma tumour. V35B antibody shows reactivity against the monocytic leukemia cell line U937, ECV304 cells and guinea pig tissue.

Immunogen

Osteoclastoma tumour

Application

Immunocytochemistry

Culture Medium

RPMI 1640 (R0883) + 2mM L-Glutamine (G7513) + 10% FBS (F2442) + HT Supplement (H0137)

Subculture Routine

Allow hybridomas to become 75% confluent prior to splitting. Seed at 3-9 x 100,000 cells/ml 5% CO₂, 37°C,

Other Notes

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UPC:

41106509

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

VL

MPN:

01080924-1VL

Temperature Control Device:

Yes

Controlled Substances and Cyanide:

Yes