Ponceau S - 50gm

Ponceau S is a stain used to visualize proteins on nitrocellulose of PVDF membranes. To create Ponceau S solution; dissolve 0.5gm of Ponceau S in 1ml of glacial acetic acid, and bring to 100ml with ddi water. To stain with Ponceau S; remove blot and rinse with ddi water, stain with Ponceau S solution for 1 to 2 minutes. Rinse for 3 to 5 minutes with ddi water. Red bands should appear against the white background. To reversal stain with Ponceau S Solution; place the nitrocellulose membrane in Ponceau S solution for 5 minutes at room temperature, destain in ddi water for 2 minutes and mark the molecular weight standard with indelible ink, finally to complete the destain soak in water for 10 minutes. Alternatively, use a 0.1N NaOH solution for 5 minutes if proteins of interest are not basic.

UPC:

41105322

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

EA

WeightUOM:

LB

MPN:

IB01100