Immobilized Protein A/G Resin, 10 x 0.2mL columns

Immobilized Protein A/G consists of recombinant protein A/G ligand covalently immobilized onto 6% highly cross-linked agarose.The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc. Protein A/G binds well to IgG subclasses but does not bind IgA, IgM or serum albumin. This makes Protein A/G an excellent tool for purification and detection of monoclonal antibodies from IgG subclasses, without interference from IgA, IgM and serum albumin. Individual subclasses of monoclonals are likely to have a stronger affinity to the chimeric Protein A/G than to either Protein A or Protein G. The Immobilized Protein A/G 1ml spin column kit is ideal for the small scale affinity purification of antibodies form a variety of samples.Each 1ml column enables purification of 1-13mg IgG from 0.5-2ml of serum or other sample. The Immobilized Protein A/G 0.2ml spin column kit is ideal for the small scale affinity purification of antibodies form a variety of samples.Each 0.2ml column enables purification of 0.1-1mg IgG from 25-500µl of serum or other sample. Specific binding and elution buffers are also available. Click here to request bulk or custom sizes . Features High binding capacity: 38mg human IgG/ml resin; >20mg sheep IgG/ml resin Ligand : Recombinant Streptococcal protein A/G lacking the albumin binding sites expressed in E. coli Bead size: 50-165μm Bead Structure: 6% highly cross-linked agarose Applications Suitable for immunoaffinity chromatography and immunoprecipitation.

UPC:

41116127

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

10

Unit of Measurement:

PK

WeightUOM:

LB

MPN:

786-840