PROTEIN A, RECOMBINANT

Protein A is a cell-wall receptor which is present on cell wall of various starins of Staphyloccocus aureus. Protein A contains four high-affinity binding sites for interacting with the Fc region of immunoglobulin Gs of various species including mouse, rabbit and human. Protein A is used for making probes for detection of antibodies and for making affinity media for purification of antibodies. Protein A (Recombinant) is recombinant protein A expressed in E. coli. It functions similarly as the native Protein A. Protein A (Recombinant) can be conjugated with reporter molecules, fluorescent dyes, enzyme markers, biotin or colloidal gold for detection of antibodies by immunochemical assays including ELISA, western blotting or Immunohistochemistry. Protein A (Recombinant) can be immobilized on a solid support such as agarose to purify monoclonal or polyclonal antibodies. Features: Source: E. coli . Molecular weight: ~47kDa; exact sequence as the native Protein A. Binds 3 IgG Isotypes. Purity: ≥ 95%, HPLC, Single major band . Form: Lyophilized powder. Application(s): Protein A (Recombinant) conjugated to reporter molecules or fluourescent dyes or enzymemarkers can be used in detection of antibody by immunochemicals assays. Protein A (Recombinant)immobilized on a solid support such as agarose is usedto purify monoclonal or polyclonal antibodies. Image By E A S (Own work)