G-Trap Protein G, 2 x 1mL columns

G-Trap™ ProteinG are prepacked ready to use columns for affinity purification of Immunoglobulins G. The column is packed with resin, which is covalently coupled to recombinant Protein G. Recombinant Protein G is coupled covalently by proprietary coupling method to highly crosslinked 6% agarose. The coupling chemistry used shows high coupling efficiency and low Protein G leaching and thus high binding efficiency. The recombinant Protein G, produced in E. coli, has been designed to genetically remove albumin and cell surface binding regions of the protein. G-Trap™ rProtein A FF, 1 ml and G-Trap™ rProtein A FF, 5 ml are also available for affinity purification of Immunoglobulins. The properties of the column and resin are listed below G-Trap™ Protein G resin Mean particle size : 90 µM Bead Structure :Highly cross-linked 6% agarose Ligand :Recombinant Streptococcal Protein G lacking the albumin binding domain produced in E. coli Binding capacity :38mg human IgG/ml resin ; >20mg sheep IgG/ml resin Maximum flow rate :1000 cm/hr Chemical Stability :Stable in all commonly used buffers pH stability : Long term: pH 3-9, short term pH 2-9 Storage :2°C to 8°C in 20% ethanol G-Trap™ Protein G, 1 ml columns Column Volume : 1 ml Columns Dimension : 0.7 x 2.5 cm Column Harware Pressure Limit : 0.5 MPa Column Hardware : Polypropylene G-Trap™ Protein G, 5ml columns Column Volume : 5ml Columns Dimension : 1.6 x 2.5 cm Column Harware Pressure Limit : 0.5 MPa Column Hardware : Polypropylene Application(s): G-Trap™ Protein G are prepacked ready to use columns for affinity purification of Immunoglobulins G