G-Trap rProtein A FF, 2 x 1mL columns

G-Trap™ rProtein A FF are prepacked ready to use columns for purification of IgG antibodies. The column is packed with fast flow resin, which is covalently coupled to recombinant Protein A. Recombinant Protein A is coupled covalently by proprietary coupling method to highly crosslinked 6% agarose. The recombinant Protein A, produced in E. coli, has been genetically engineered to enhance its binding capacity to the antibodies G-Trap™ Protein G, 1 ml and G-Trap™ Protein G, 5 ml are also available for affinity purification of Immunoglobulins G. The key characteristics of the recombinant Protein A resin or matrix used in G-Trap™ rProtein A FFand the column properties are listed below G-Trap™ rProtein A resin Mean particle size :90 µM Bead Structure :Highly cross-linked 6% agarose Ligand : Recombinant Staphylococcal Protein A from E.coli Binding capacity: >40 mg Human IgG/ ml resin Recommended working flow rate :1 ml/min and 5 ml/min for 1 ml and 5 ml column respectively. Chemical Stability :Stable in all commonly used buffers pH stability: Long term: pH 3-9, short term pH 2-9 Storage: 2°C to 8°C in 20% ethanol G-Trap™ rProtein A, 1 ml columns Column Volume : 1 ml Columns Dimension : 0.7 x 2.5 cm Column Harware Pressure Limit : 0.5 MPa Column Hardware : Polypropylene (Biocompatible) G-Trap™ rProtein A, 5 ml columns Column Volume : 5 ml Columns Dimension : 1.6 x 2.5 cm Column Harware Pressure Limit : 0.5 MPa Column Hardware : Polypropylene (Biocompatible) Application (s): G-Trap™ rProtein A FF are prepacked ready to use columns used for affinity purification of IgG antibodies.