Pseudomonas Isolation Agar Base is for selective isolation and identification of Pseudomonas aeruginosa from clinical and nonclinical specimens. Pack size is 2.5KG

Pseudomonas aeruginosa is an important human pathogen commonly found in nosocomial infections. It successfully combines adaptability to a variety of moist environments with a collection of potent virulence factors (1). Pseudomonas infections usually occur at any site where moisture tends to accumulate e. g. tracheostomies, in-dwelling catheters, burns, the external ear and weeping cutaneous wounds (2). Pseudomonas Isolation Agar Base, used for the selective isolation and identification of P. aeruginosa , is a modification of Medium A, originally formulated by King, Ward and Raney (3). The medium contains pigment-enhancing components and the selective agents, triclosan (4) which selectively inhibits non-pseudomonads. The pigment-enhancers i.e. potassium sulphate and magnesium chloride enhance the blue or blue-green pigment production by P. aeruginosa , thus aiding in its identification. Peptic digest of animal tissue provides nitrogenous compounds and other essential growth nutrients. Glycerol is a source of energy and promotes pyocyanin i.e. pigment production which is characteristic of Pseudomonas (5, 6). Potassium sulphate and magnesium chloride enhance pyocyanin production. Triclosan (7) selectively inhibits gram-positive and gram-negative bacteria but Pseudomonas species are resistant to it. Some pyocyanin producing strains may also produce small amounts of fluorescein, resulting in the production of a blue-green to green pigment. Presumptive Pseudomonas should be further confirmed by performing biochemical tests, as some strains of Pseudomonas do not produce pyocyanin (8). Directions: Suspend 45.03 grams in 1000 ml distilled water containing 20 ml glycerol. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and pour into sterile Petri plates.

UPC:

41106202

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

EA

WeightUOM:

LB

MPN:

M406-2.5KG

Controlled Substances and Cyanide:

Yes