Cell Line Origin
Rat embryo
Cell Line Description
RAT-2 was derived from a subclone of a 5′-bromo-deoxyuridine (BUdR)-resistant strain of the Fischer rat 3T3-like cell line RAT-1. RAT-2 lacks appreciable levels of nuclear thymidine kinase, is highly transfectable by exogenous DNA and is phenotypically normal.
Culture Medium
DMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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biological source: rat embryo. growth mode: Adherent. karyotype: Not specified. morphology: Fibroblast-like. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 11131607
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 94050409-1VL