Rb69 gene 32 protein

Rb69 gene 32 protein is a single-stranded DNA-binding protein encoded by gene 32 of Rb69 phage, with a molecular weight of 34 kDa. It is essential for DNA replication and repair of Rb69 phage. The protein binds cooperatively and stabilizes transiently formed single-stranded DNA regions, playing an important structural role during Rb69 phage DNA replication. It is also widely used to stabilize and label single-stranded DNA regions for observing intracellular DNA structure under electron microscopy. Rb69 gene 32 protein can enhance restriction endonuclease digestion efficiency, improve reverse transcription efficiency in RT-PCR, stimulate DNA polymerase activity, and is applicable to Recombinase Polymerase Amplification (RPA) reactions.ApplicationsIncrease reverse transcription yield and extension capacity in RT-PCR.Improve target fragment yield and specificity in PCR of soil samples.Stabilize and label ssDNA structures.Quality Control1. Protein PurityDetermined by SDS-PAGE; purity ≥ 95%.2. Endonuclease Activity Assay10 μg of Rb69 gene 32 protein was incubated with 200 ng supercoiled plasmid DNA at 37 °C for 4 h. Agarose gel electrophoresis showed less than 10% of plasmid DNA was converted into nicked or linear form.3. Non-specific Nuclease Activity Assay10 μg of the protein was incubated with 15 ng double-stranded DNA fragment at 37 °C for 16 h. No alteration of dsDNA substrate was observed by agarose gel electrophoresis.4. RNase Activity Assay10 μg of the protein was incubated with 500 ng total RNA at 37 °C for 1 h. Agarose gel electrophoresis confirmed over 90% of RNA remained intact.5. Host Genomic DNA ResidueQuantitative real-time PCR with E. coli 16S rDNA-specific primers and probes was used to detect 10 μg protein; residual E. coli genomic DNA was less than 10 copies.Inactivation ConditionIncubation at 65 °C for 20 min completely inactivates the protein.

Specifications & Purity: Rb69 gene 32 protein is a single-stranded DNA-binding protein encoded by gene 32 of Rb69 phage, with a molecular weight of 34 kDa. It is essential for DNA replication and repair of Rb69 phage. The protein binds cooperatively and stabilizes transiently formed single-stranded DNA regions, playing an important structural role during Rb69 phage DNA replication. It is also widely used to stabilize and label single-stranded DNA regions for observing intracellular DNA structure under electron microscopy. Rb69 gene 32 protein can enhance restriction endonuclease digestion efficiency, improve reverse transcription efficiency in RT-PCR, stimulate DNA polymerase activity, and is applicable to Recombinase Polymerase Amplification (RPA) reactions.ApplicationsIncrease reverse transcription yield and extension capacity in RT-PCR.Improve target fragment yield and specificity in PCR of soil samples.Stabilize and label ssDNA structures.Quality Control1. Protein PurityDetermined by SDS-PAGE; purity ≥ 95%.2. Endonuclease Activity Assay10 μg of Rb69 gene 32 protein was incubated with 200 ng supercoiled plasmid DNA at 37 °C for 4 h. Agarose gel electrophoresis showed less than 10% of plasmid DNA was converted into nicked or linear form.3. Non-specific Nuclease Activity Assay10 μg of the protein was incubated with 15 ng double-stranded DNA fragment at 37 °C for 16 h. No alteration of dsDNA substrate was observed by agarose gel electrophoresis.4. RNase Activity Assay10 μg of the protein was incubated with 500 ng total RNA at 37 °C for 1 h. Agarose gel electrophoresis confirmed over 90% of RNA remained intact.5. Host Genomic DNA ResidueQuantitative real-time PCR with E. coli 16S rDNA-specific primers and probes was used to detect 10 μg protein; residual E. coli genomic DNA was less than 10 copies.Inactivation ConditionIncubation at 65 °C for 20 min completely inactivates the protein.

Grade: Recombinant;BioReagent

Storage Temperature: Store at -20°C;Avoid repeated freezing and thawing

Shipped in: Ice chest + Ice pads

UPC:

51172437

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

EA

WeightUOM:

LB

MPN:

R1522662-1mg

Country of Origin:

China

Product Size:

1mg

Harmonized Tariff Code:

2931.90.90