Recombinant Aprotinin

Aprotinin is a competitive inhibitor of serine proteases, which can inhibit the activity of trypsin, chymotrypsin, kininogenase, etc. Aprotinin can form a stable complex with proteases to block the active sites of the enzymes. Recombinant aprotinin has the same enzymatic properties as animal-derived aprotinin, and can replace animal-derived aprotinin in various biotechnological processes, such as inhibiting the activity of serine proteases in recombinant protein production and cell culture.Mechanism of Action: Aprotinin can form a stable complex with proteases to block the active sites of the enzymes. Most aprotinin-protease complexes dissociate under conditions of pH < 3.0. Aprotinin binds to proteases in an equimolar effective ratio.Amino Acid Sequence: Recombinant aprotinin is expressed in recombinant E. coli and purified through multiple column chromatography steps; its amino acid sequence is identical to that of aprotinin derived from bovine lung.Enzymatic Properties: Recombinant aprotinin has the same enzymatic properties as animal-derived aprotinin, and can replace animal-derived aprotinin in various biotechnological processes.Usage Instructions: Aprotinin binds to proteases in an equimolar effective ratio; the recommended binding pH is > 6.0, and no binding occurs under conditions of pH < 3.0.It can be directly dissolved in 0.9% NaCl; after dissolution, it can be stored at -20°C.Product Advantages: Animal-origin-free: Produced via recombination, no exogenous virus contamination, and no animal-derived raw materials are used in the production process.Stable quality: Mass production enables stable and continuous batch production; there is no batch-to-batch variation, and the quality is stable.High purity: Host protein residues are below the limit requirements for biological products.Lyophilized powder: Easy to store and transport.Compliant with regulatory requirements: Production equipment and environment meet relevant regulatory requirements; the production process fully follows the NSF ISO 9001:2015 quality system and complies with GMP guidelines.Complete quality documentation: Relevant regulatory support documents can be provided according to customer needs.

Specifications & Purity: Aprotinin is a competitive inhibitor of serine proteases, which can inhibit the activity of trypsin, chymotrypsin, kininogenase, etc. Aprotinin can form a stable complex with proteases to block the active sites of the enzymes. Recombinant aprotinin has the same enzymatic properties as animal-derived aprotinin, and can replace animal-derived aprotinin in various biotechnological processes, such as inhibiting the activity of serine proteases in recombinant protein production and cell culture.Mechanism of Action: Aprotinin can form a stable complex with proteases to block the active sites of the enzymes. Most aprotinin-protease complexes dissociate under conditions of pH < 3.0. Aprotinin binds to proteases in an equimolar effective ratio.Amino Acid Sequence: Recombinant aprotinin is expressed in recombinant E. coli and purified through multiple column chromatography steps; its amino acid sequence is identical to that of aprotinin derived from bovine lung.Enzymatic Properties: Recombinant aprotinin has the same enzymatic properties as animal-derived aprotinin, and can replace animal-derived aprotinin in various biotechnological processes.Usage Instructions: Aprotinin binds to proteases in an equimolar effective ratio; the recommended binding pH is > 6.0, and no binding occurs under conditions of pH < 3.0.It can be directly dissolved in 0.9% NaCl; after dissolution, it can be stored at -20°C.Product Advantages: Animal-origin-free: Produced via recombination, no exogenous virus contamination, and no animal-derived raw materials are used in the production process.Stable quality: Mass production enables stable and continuous batch production; there is no batch-to-batch variation, and the quality is stable.High purity: Host protein residues are below the limit requirements for biological products.Lyophilized powder: Easy to store and transport.Compliant with regulatory requirements: Production equipment and environment meet relevant regulatory requirements; the production process fully follows the NSF ISO 9001:2015 quality system and complies with GMP guidelines.Complete quality documentation: Relevant regulatory support documents can be provided according to customer needs.

Grade: Bioactive;Recombinant;ActiBioPure™;High Performance;EnzymoPure™

Molecular Formula: C284H432N84O79S7

Molecular Weight: 6511.44

EC Number: 232-994-9

PubChem CID: 22833874

Isomeric SMILES: CCC(C)C1C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC2CSSCC3C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(CSSCC(C(=O)NC(C(=O)NC(C(=O)N4CCCC4C(=O)N5CCCC5C(=O)NC(C(=O)NC(C(=O)NCC(=O)N6CCCC6C(=O)NC(CSSCC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)N3)CC(=O)O)CCC(=O)O)C)CO)CCCCN)CC7=CC=CC=C7)CC(=O)N)CC(=O)N)CCCNC(=N)N)CCCCN)C)CCCNC(=N)N)NC(=O)CNC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC2=O)CCC(=O)N)C(C)O)CC8=CC=CC=C8)C(C)C)CC9=CC=C(C=C9)O)C(=O)NC(C(=O)NC(C(=O)NC(C(=O)N1)CCCNC(=N)N)C)CCCCN)C(C)O)CC1=CC=C(C=C1)O)CCC(=O)O)CC(C)C)NC(=O)C(CC1=CC=CC=C1)NC(=O)C(CC(=O)O)NC(=O)C1CCCN1C(=O)C(CCCNC(=N)N)N)C(=O)NCC(=O)NCC(=O)NC(C)C(=O)O)C(C)O)CCCNC(=N)N)CCSC)CC(C)C)C)CCCCN)C)CC(=O)N)CC1=CC=C(C=C1)O)CC1=CC=CC=C1)CC1=CC=C(C=C1)O)CCCNC(=N)N)C(C)CC

Storage Temperature: Store at -20°C

Shipped in: Ice chest + Ice pads

More Information: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/R141091-SCI_a5d4626e5351cdc093a190ca6d4249bb.pdf

UPC:

51281603

Weight:

1.06 Ounces

HazmatClass:

Yes

Quantity:

1

Unit of Measurement:

EA

WeightUOM:

LB

MPN:

R141091-1mg

Country of Origin:

China

CAS:

9087-70-1

Product Size:

1mg

Harmonized Tariff Code:

2931.90.90

Hazard Statement Codes:

H302;H317;H334

Precautionary Statement Codes:

P261;P264;P270;P272;P280;P284;P321;P330;P302+P352;P304+P340;P333+P313;P362+P364;P501;P301+P317;P342+P316