Description: Staphylococcus aureus alpha hemolysin (Hla), also known as alpha toxin (α-toxin), is expressed in E. coli as a recombinant protein without TAG. The theoretical molecular weight of the protein is approximately 33 kDa. Size: 100 µg is supplied in phosphate buffered saline at a concentration of 0.857 mg/mL Endotoxin: 8.029 Endotoxin units/mg Relevance: Hla is produced by S. aureus and is a major cytotoxic agent. It is expressed as a monomer that binds to the membrane of susceptible cells. Subunits then oligomerize to form heptameric rings with a central pore through which cellular contents leak. The mode of action of S. aureus Hla is likely by osmotic lysis Recommended Dilutions: ELISA: Assay-dependent dilution. WB: Assay-dependent dilution; internal QC demonstrates detection of Hla protein using IBT’s mouse anti-Alpha Toxin (6C12) monoclonal antibody (cat# 0210-005) in Western blotting. Cytotoxicity assay: The protein is active in functional lysis assays with rabbit red blood cells. The protein can be also used in serology assay as coating antigen to detect antibodies against Hla. Storage: -80°C Intended for research use only, not for human, therapeutic, or diagnostic applications.
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- UPC:
- 51201801
- Condition:
- New
- Availability:
- 3 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 1401-002
- Temperature Control Device:
- Yes
