Recombinant LukE (tag free), 100ug

Description: : Purified, Staphylococcus aureus recombinant leukocidin-E (rLukE). The rLukE (tag free) is expressed in E. coli and purified by FPLC. The theoretical molecular weight of the protein is 34,819 Daltons Size: 100 µg of protein is supplied in PBS at a concentration of 2.417 mg/mL. Protein demonstrates a molecular weight of approximately 35 kDa. Relevance: This protein may be used in functional toxicity assays in combination with rLukD, or as a control protein in ELISA assays or Western blotting when detecting toxins produced by different strains of S. aureus. Recommended Dilutions: ELISA: Assay-dependent dilution WB: Assay-dependent dilution; internal QC demonstrates detection of 100 ng of rLukE protein using anti-LukS-PV polyclonal antibody (cat# 04-0009) by Western blot analysis. Cytotoxicity assay: Cytotoxicity can be detected in human neutrophils when used in combination with rLukD in a concentration range of 25-800 nM. Storage: 2-3 weeks at -20°C, -80°C long term. Intended for research use only, not for human, therapeutic, or diagnostic applications.

UPC:

51283203

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

EA

WeightUOM:

LB

MPN:

0530-004