Description: Recombinant TSST-1 expressed in E.coli and purified to >90% using chromatographic methods. Size: 100 µg of protein is supplied in PBS + 10% Glycerol at a concentration of 1.3 mg/mL. The theoretical molecular weight of the TSST-1 is 22,204 Daltons. Relevance: TSST-1 is a pyrogenic toxin superantigen secreted by Staphylococcus aureus. TSST-1 is a powerful activator of T-lymphocytes resulting in substantial cytokine release that can cause severe pathological consequences. rTSST-1 can be utilized as a control in ELISA and Western blot assays, as a potent mitogen, or as an activator of cytokines in Peripheral Blood Mononuclear Cells (PBMC). Recommended Dilutions: ELISA: Assay-dependent dilution. WB: Assay-dependent dilution; internal QC demonstrates strong detection of 1 ng of TSST-1. PBMC Stimulation assay: Stimulation of PBMCs to produce Interferon γ by TSST-1 can be achieved with 0.1 ng/mL. Storage: 2-3 weeks at -20°C, -80°C long term Intended for research use only, not for human, therapeutic, or diagnostic applications.
- UPC:
- 41122409
- Condition:
- New
- Availability:
- 3 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 1402-001
