SDS-PAGE Sample Loading Buffer (Non-reduced, 5X) (C007B-585301)

SDS-PAGE Sample Loading Buffer (Non-reduced, 5X) is a 5-fold concentrated SDS-PAGE gel electrophoresis sample loading buffer with bromophenol blue as the dye. It has the function of precipitating protein samples into the gel pores and indicating the movement position of protein samples in the gel. At the same time, the buffer does not contain reducing agents such as DTT/B-mercaptoethanol, which does not affect the disulfide bond in the chain and the disulfide bond between the chains of protein molecules. But the solution contains SDS type denaturing agents. Suitable for protein sample preparation and loading in denatured non reduced SDS-PAGE.Precautions:1. Before adding the sample, thaw it at room temperature or 37-40 °C for a few minutes and gently shake well to ensure even mixing of the solution;2. This product is only used for scientific research and cannot be used for human experiments or treatments;3. For your own safety, please take protective measures such as wearing lab coats and gloves before using the reagents.Instructions for Use:1. Reagent preparation1.1 Mix the protein tracer loading buffer (non reducing, 5x) with the protein sample in a ratio of 1:4;2. Protein sample processing2.1 Heat the protein sample in a boiling water bath for 3-5 minutes;2.2 After sufficient denaturation of the protein sample, cool it to room temperature and centrifuge at less than 3000rpm for 30 seconds:3. Protein loading and electrophoresis3.1 After centrifugation, take appropriate amount of supernatant with micro sampler and directly add it into the SDS-PAGE gel sampling hole;3.2 Conduct routine electrophoresis. Generally, stop electrophoresis when the dye reaches 0.5cm-1cm from the bottom of gel.

Specifictaions: BioReagent, for western blot, for PAGE

UPC:

41105310

Condition:

New

Weight:

2.64 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

EA

WeightUOM:

LB

MPN:

S767014-5×5ml

Product Size:

5×5ml