Sialic Acid (SA) Content Assay Kit (UV Micro Method) (C008B-266648)

Sialic acid, also known as neuraminic acid, is widely present in animal tissues and microorganisms. It is an important component of glycoproteins and glycolipids on cell membranes, located at the terminal ends, and participates in various physiological functions on the cell surface. In malignant tumors, the structure and content of glycoproteins and glycolipids on the cell surface undergo significant changes, causing large amounts of sialic acid to detach from cancer cells and enter the bloodstream, leading to elevated serum sialic acid levels. Determination of sialic acid content is of great significance for the study of certain congenital metabolic disorders and Behcet syndrome. Elevated sialic acid is also one of the risk factors for cardiovascular diseases. Sialic acid is acted upon by neuraminidase to form N‑acetylneuraminic acid, which is then converted into pyruvate and N‑acetylmannosamine by N‑acetylneuraminate aldolase. Pyruvate is converted intoPhospholipids are hydrolyzed by phospholipase D to produce choline and phosphatidic acid. Choline is oxidized by choline oxidase to generate hydrogen peroxide, which reacts with 4‑aminoantipyrine and DAOS to form a blue dye. This dye has a maximum absorption peak at 600 nm, and the absorption intensity is proportional to the phospholipid content in the sample. lactate and NAD⁺ by lactate dehydrogenase (LDH) in the presence of NADH. The sialic acid concentration in the sample can be obtained by measuring the rate of decrease in NADH absorbance.

Specifications & Purity: Sialic acid, also known as neuraminic acid, is widely present in animal tissues and microorganisms. It is an important component of glycoproteins and glycolipids on cell membranes, located at the terminal ends, and participates in various physiological functions on the cell surface. In malignant tumors, the structure and content of glycoproteins and glycolipids on the cell surface undergo significant changes, causing large amounts of sialic acid to detach from cancer cells and enter the bloodstream, leading to elevated serum sialic acid levels. Determination of sialic acid content is of great significance for the study of certain congenital metabolic disorders and Behcet syndrome. Elevated sialic acid is also one of the risk factors for cardiovascular diseases. Sialic acid is acted upon by neuraminidase to form N‑acetylneuraminic acid, which is then converted into pyruvate and N‑acetylmannosamine by N‑acetylneuraminate aldolase. Pyruvate is converted intoPhospholipids are hydrolyzed by phospholipase D to produce choline and phosphatidic acid. Choline is oxidized by choline oxidase to generate hydrogen peroxide, which reacts with 4‑aminoantipyrine and DAOS to form a blue dye. This dye has a maximum absorption peak at 600 nm, and the absorption intensity is proportional to the phospholipid content in the sample. lactate and NAD⁺ by lactate dehydrogenase (LDH) in the presence of NADH. The sialic acid concentration in the sample can be obtained by measuring the rate of decrease in NADH absorbance.

Grade: BioReagent

Storage Temperature: Store at 2-8°C;Protected from light

Shipped in: Wet ice

UPC:

51302001

Weight:

1.09 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

ST

WeightUOM:

LB

MPN:

S1522114-96T

Country of Origin:

China

Product Size:

96T

Harmonized Tariff Code:

2931.90.90