Solubility Enhancer for Lipophilic Fluorescent Probes (C007B-566201)

Solubility Enhancer for Lipophilic Fluorescent Probes is a new type of polymeric nonionic surfactant, commonly used to enhance the water solubility of lipophilic fluorescent probes or dyes and improve their cell permeability. This product is 500X concentrated in DMSO and can be used directly in cell experiments.Lipophilic fluorescent probes, such as DiO (cell membrane green fluorescent probe) , DiI (cell membrane red fluorescent probe) and DiD (cell membrane far-infrared fluorescent probe) , may precipitate when preparing the staining working solution due to their low water solubility. This product can effectively solve the precipitation problem and improve the cell permeability of lipophilic fluorescent probes, leading to faster cell membrane staining with brighter fluorescence and lower staining background.Precautions：This product must be completely dissolved before use. If crystal precipitates occur in this product, heat at 37-50℃ to promote dissolution, which has no effect on subsequent use. This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.Instructions for Use：1.Use this product to improve the water solubility of lipophilic fluorescent probes if precipitation frequently occurs in the their working solutions. The addition of this product into the working solution of lipophilic fluorescent probes at a ratio of 1:500 can effectively promote the dissolution of lipophilic fluorescent probes and improve their staining results.2.The following protocol is provided for the preparation of DiO working solution with this product. Preparation of other lipophilic fluorescent probes can be referred as appropriate. a.Amount of DiO working solution required: For per well of 6, 12, 24 and 96-well plates, the required amount of DiO working solution is 1~2ml, 0.5~1ml, 300~500μl and 50~100μl, respectively. For samples to be examined by flow cytometry, prepare 0.5ml of DiO working solution for each sample is. For sections, use 100-200μl of DiO working solution per section, depending on the size of sections. b.Preparation of DiO stock solution (500X): DiO can be dissolved in absolute ethanol, DMSO and DMF, with a solubility over 10 mg/ml (11.3 mM) in DMSO. Heat and ultrasonicate the solution appropriately when DiO is difficult to dissolve. A final working concentration of DiO at 5-10μM is most commonly used. DiO can be prepared into a stock solution as required. For example, prepare a 500X concentrated DiO stock solution (5mM) if a final working concentration at 10μM will be needed for staining. c.Preparation of DiO working solution: Prepare an appropriate amount of DiO working solution based on the number of samples. For samples to be examined by flow cytometry, each sample will require 0.5ml of DiO working solution, and prepare sufficient DiO working solution for different numbers of samples as follows: Reagent 2Sample 20Samples 200Samples DiO (500X) 2μl 20μl 200μl Solubility Enhancer(500X) 2μl 20μl 200μl AssayBuffer 996μl 9.96ml 99.6ml TotalVolume 1ml 10ml 100ml Note 1: DiO (500X) and Solubility Enhancer (500X) must be mixed well before adding the Assay Buffer. The Aassay Buffer can be normal cell culture medium, serum-free culture medium, HBSS , PBS , or 's Extracellular Solution . The Extracellular Solution can maintain cells in normal condition for a period of time by providing some nutrition to cells, and thus is usually better than PBS or HBSS. However, the use of normal cell culture medium may lead to a decrease in the staining effect, which can be avoided by extending the staining time or increasing the dye concentration appropriately.Note 2: The optimal staining concentration varies slightly from cell to cell, which should be optimized for different cell lines and experimental systems. When the final concentration of DiO is increased, the amount of Solubility Enhancer remains the same.d.Stain cells using an appropriate staining method with DiO fluorescent probe.Related Products:Cat. No.Product NamePack Size C1036 DiI (cell membrane red fluorescent probe) 10mg C1038 DiO (cell membrane green fluorescent probe) 10mg C1039-10mg DiD (cell membrane far-infrared fluorescent probe) 10mg C1990-1ml Solubility Enhancer for Lipophilic Fluorescent Probes (500X) 1ml C1990-5ml Solubility Enhancer for Lipophilic Fluorescent Probes (500X) 5ml

Specification: 500X

UPC:

41115701

Condition:

New

Weight:

0.07 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

EA

WeightUOM:

LB

MPN:

S752156-1ml

Product Size:

1ml