SPDP-C6-NHS ester

The amine-reactive portion of SPDP reagents is the N-hydroxysuccinimide (NHS) ester. Reactions are most commonly performed in phosphate, carbonate/bicarbonate, or borate buffers at pH 7-8. Other buffers can be used provided they do not contain primary amines (or thiols or disulfide reducing reagents – see discussion of the sulfhydryl reactivity). The rate of reaction and degradation by hydrolysis increases with increasing pH; for example, the half-life of the NHS ester is several hours at pH 7 and less than 10 minutes at pH 9. NHS-ester reagents like SPDP and LC-SPDP have limited aqueous solubility and must be dissolved in organic solvent before adding them to a reaction mixture. Sulfo-NHS-ester reagents like Sulfo-LC-SPDP are water-soluble and can be added directly to aqueous reaction mixtures.Features and advantages:Reactive groups: NHS ester and pyridyldithiolReactive towards: amino and sulfhydryl groupsReleases a detectable by-product when reacted with a free sulfhydryl group; by measuring the release of pyridine-2-thione at 343 nm, the reaction can be easily followedDisulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5Spacer arm is also easily cleaved using reducing SDS-PAGE sample loading bufferCleavable crosslinker allows separation of crosslinked productsWater-insoluble (dissolve first in DMF or DMSO)SPDP crosslinker is membrane permeable, so crosslinking can be done inside the cellsCompare to other varieties of SPDP-type reagents, including pegylated forms

Non-PEG linker

Specifications & Purity: The amine-reactive portion of SPDP reagents is the N-hydroxysuccinimide (NHS) ester. Reactions are most commonly performed in phosphate, carbonate/bicarbonate, or borate buffers at pH 7-8. Other buffers can be used provided they do not contain primary amines (or thiols or disulfide reducing reagents – see discussion of the sulfhydryl reactivity). The rate of reaction and degradation by hydrolysis increases with increasing pH; for example, the half-life of the NHS ester is several hours at pH 7 and less than 10 minutes at pH 9. NHS-ester reagents like SPDP and LC-SPDP have limited aqueous solubility and must be dissolved in organic solvent before adding them to a reaction mixture. Sulfo-NHS-ester reagents like Sulfo-LC-SPDP are water-soluble and can be added directly to aqueous reaction mixtures.Features and advantages:Reactive groups: NHS ester and pyridyldithiolReactive towards: amino and sulfhydryl groupsReleases a detectable by-product when reacted with a free sulfhydryl group; by measuring the release of pyridine-2-thione at 343 nm, the reaction can be easily followedDisulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5Spacer arm is also easily cleaved using reducing SDS-PAGE sample loading bufferCleavable crosslinker allows separation of crosslinked productsWater-insoluble (dissolve first in DMF or DMSO)SPDP crosslinker is membrane permeable, so crosslinking can be done inside the cellsCompare to other varieties of SPDP-type reagents, including pegylated forms

Molecular Formula: C18H23N3O5S2

Molecular Weight: 425.52

EC Number: 968-854-5

PubChem CID: 157808

Isomeric SMILES: C1CC(=O)N(C1=O)OC(=O)CCCCCNC(=O)CCSSC2=CC=CC=N2

Storage Temperature: Store at -20°C

Shipped in: Ice chest + Ice pads

UPC:

51273913

Weight:

1.06 Ounces

HazmatClass:

Yes

Quantity:

1

Unit of Measurement:

EA

WeightUOM:

LB

MPN:

S595683-5mg

Country of Origin:

China

CAS:

158913-22-5

Product Size:

5mg

Harmonized Tariff Code:

2921.19.61