Cell Line Origin
Mouse BALB/3T3 clone A31
Cell Line Description
Derived by SV40 transformation of the line BALB/3T3 clone A31. The cells exhibit a high saturation density, lack contact inhibition and form tumours in appropriate hosts.
Application
Transformation, tumourigenicity and virus studies: SV40
Culture Medium
DMEM + 2mM Glutamine + 10% New Born Calf Serum (NBCS) or (5% NBCS + 5% Foetal Bovine Serum (FBS)).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1x1,000 to 3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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biological source: mouse embryo. growth mode: Adherent. karyotype: 2n : 40, modal No 41. morphology: Fibroblast. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 11131607
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 90030801-1VL