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INTACT GENOMICS

T7 Endonuclease I - 1,250 units

T7 Endonuclease I, a stable homodimer of identical 149 amino acid subunits (1) is the product of a recombinant gene in E. coli .

Description

T7 Endonuclease I, a stable homodimer of identical 149 amino acid subunits (1) is the product of a recombinant gene in E. coli . Double-stranded breaks (DSBs) generated by CRISPR/TALEN at desired target sites can be PCR-amplified, and the PCR products can be denatured and re-annealed to form mismatched DNA. If the mismatched DNA length position is more than 1 bp, T7 endonuclease I can recognize and cleave it. It is useful for quantitatively estimating the nuclease-induced mutation frequency of gene edited cells. Product Includes: • T7 Endonuclease I • 10x T7 Endonuclease I reaction buffer

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Specifications
UPC:
51283204
Condition:
New
Weight:
1.00 Ounces
HazmatClass:
No
Quantity:
1
Unit of Measurement:
PK
WeightUOM:
LB
MPN:
3612
Temperature Control Device:
Yes
Catalog No. C003B-531241
Price: $517.55
List Price: $575.05
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