TBS-T Buffer Instant Granules (C008B-270243)

TBS-T appears as white to off-white instant granules. Each pouch of TBS-T instant granules is sufficient to prepare 1 L of 1× TBS-T buffer, featuring simple and convenient operation.The main components of TBS-T are sodium chloride, potassium chloride, Tris buffer salts and Tween-20. The 1× TBS-T buffer contains 137 mM sodium chloride, 2.7 mM potassium chloride, 25 mM Tris buffer salts and 0.05% Tween-20. The pH value of the prepared 1× solution is 7.4±0.15 at 25℃.As a widely used biological buffer, TBS-T is mainly applied in immunohistochemistry, in situ hybridization (ISH), enzyme-linked immunosorbent assay (ELISA) and Western blotting. It is commonly used as a washing buffer, and also frequently for antibody dilution and blocking solution preparation.Protocol1. Measure approximately 600 mL of distilled water into a beaker, and place a magnetic stir bar in the beaker.2. Place the beaker on a magnetic stirrer. Slowly add the entire contents of one pouch of TBS-T instant granules, and stir until completely dissolved.3. Add distilled water to the TBS-T solution from Step 2 to a final volume of 1 L to obtain 1× working solution.Preparation Instructions for Instant Granules

Specifications & Purity: TBS-T appears as white to off-white instant granules. Each pouch of TBS-T instant granules is sufficient to prepare 1 L of 1× TBS-T buffer, featuring simple and convenient operation.The main components of TBS-T are sodium chloride, potassium chloride, Tris buffer salts and Tween-20. The 1× TBS-T buffer contains 137 mM sodium chloride, 2.7 mM potassium chloride, 25 mM Tris buffer salts and 0.05% Tween-20. The pH value of the prepared 1× solution is 7.4±0.15 at 25℃.As a widely used biological buffer, TBS-T is mainly applied in immunohistochemistry, in situ hybridization (ISH), enzyme-linked immunosorbent assay (ELISA) and Western blotting. It is commonly used as a washing buffer, and also frequently for antibody dilution and blocking solution preparation.Protocol1. Measure approximately 600 mL of distilled water into a beaker, and place a magnetic stir bar in the beaker.2. Place the beaker on a magnetic stirrer. Slowly add the entire contents of one pouch of TBS-T instant granules, and stir until completely dissolved.3. Add distilled water to the TBS-T solution from Step 2 to a final volume of 1 L to obtain 1× working solution.Preparation Instructions for Instant Granules

Grade: BioReagent

Storage Temperature: Room temperature

Shipped in: Normal

More Information: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/T397917-SCI_c2d630bdec4e0e84f5a8b14b5838301b.pdf

UPC:

41141807

Weight:

370.38 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

EA

WeightUOM:

LB

MPN:

T397917-10×1EA

Country of Origin:

China

Product Size:

10×1EA

Harmonized Tariff Code:

2931.90.90