Time-resolved fluorescent microspheres

This product is a polystyrene microsphere containing rare earth element EU. It features a large Stokes shift, long fluorescence lifetime, and strong detection signal. Compared to conventional fluorescence, this product exhibits higher fluorescence signal intensity, making it more suitable for the development and production of high-sensitivity chromatography quantitative projects.Usage1、Take out the microsphere stock solution and subject it to ultrasonic dispersion. 2、 Microsphere activation: Add EDC and NHS activation solution and react for 0.5~1h.Taking the labeling of a 200ul volume as an example: Take 20ul (10mg/ml) of time-resolved fluorescent microspheres and add them to 180ul of 0.05M MES pH5.5 buffer containing 0.05% TW20. After shaking and mixing, add 2ul NHS (75 mg/ml) and 2ul EDC (50 mg/ml), and mix and shake for a reaction time of 0.5h. (Note: Both EDC and NHS are dissolved in 0.05M MES pH5.5 buffer.)3、Resuspension: Centrifuge, remove the supernatant, and then resuspend with an appropriate buffer solution, followed by ultrasonic dispersion. Centrifugation conditions: centrifuge at 10,000 rpm for 10 minutes, remove the supernatant, add 200 μl of 0.05M MES5.5 for resuspension, and then perform ultrasonic dispersion. (Centrifugation conditions should be adjusted appropriately according to changes in reaction volume, and the labeling buffer should be adjusted as needed.) 4、Conjugation: Add an appropriate amount of antibodies, antigens, nucleic acids, etc. to the above solution, and shake the reaction for 1-2 hours. If aggregation occurs during the reaction, perform ultrasonic dispersion. Add 150 μg/ml antibody and conjugate for 1 hour. (If independent labeling is required, use a DNP-BSA labeling concentration of 150 μg/ml and react for another 1 hour.)5、Blocking: Add an appropriate amount of blocking solution and shake at room temperature for 0.5~1h. Add 100ul of 5% BSA in Tris solution (0.1M, pH 8.0) and mix vertically at room temperature for 0.5h (blocking). 6、Resuspending: Centrifuge, remove the supernatant, and add an appropriate amount of resuspension solution to resuspend to a quantitative concentration for use. Centrifuge at 10000prm for 10min, remove the supernatant, add 500ul of resuspension solution to dilute to 0.4mg/ml, ultrasonicate for dispersion, and store at 4℃;Precautions1.Inappropriate use of buffer solution may lead to aggregation of markers. It is recommended to add ultrasonic operation during the marking process to disperse the microspheres. 2.Centrifugation conditions should be adjusted accordingly with changes in centrifuge volume. 3.Fluorescent microspheres need to be ultrasonically dispersed before use. 4.Coupling efficiency is affected by pH, temperature, molecular weight, and salt concentration. It is recommended to optimize the reaction conditions. 5. Waste should be disposed of as hazardous waste.

Specifications & Purity: This product is a polystyrene microsphere containing rare earth element EU. It features a large Stokes shift, long fluorescence lifetime, and strong detection signal. Compared to conventional fluorescence, this product exhibits higher fluorescence signal intensity, making it more suitable for the development and production of high-sensitivity chromatography quantitative projects.Usage1、Take out the microsphere stock solution and subject it to ultrasonic dispersion. 2、 Microsphere activation: Add EDC and NHS activation solution and react for 0.5~1h.Taking the labeling of a 200ul volume as an example: Take 20ul (10mg/ml) of time-resolved fluorescent microspheres and add them to 180ul of 0.05M MES pH5.5 buffer containing 0.05% TW20. After shaking and mixing, add 2ul NHS (75 mg/ml) and 2ul EDC (50 mg/ml), and mix and shake for a reaction time of 0.5h. (Note: Both EDC and NHS are dissolved in 0.05M MES pH5.5 buffer.)3、Resuspension: Centrifuge, remove the supernatant, and then resuspend with an appropriate buffer solution, followed by ultrasonic dispersion. Centrifugation conditions: centrifuge at 10,000 rpm for 10 minutes, remove the supernatant, add 200 μl of 0.05M MES5.5 for resuspension, and then perform ultrasonic dispersion. (Centrifugation conditions should be adjusted appropriately according to changes in reaction volume, and the labeling buffer should be adjusted as needed.) 4、Conjugation: Add an appropriate amount of antibodies, antigens, nucleic acids, etc. to the above solution, and shake the reaction for 1-2 hours. If aggregation occurs during the reaction, perform ultrasonic dispersion. Add 150 μg/ml antibody and conjugate for 1 hour. (If independent labeling is required, use a DNP-BSA labeling concentration of 150 μg/ml and react for another 1 hour.)5、Blocking: Add an appropriate amount of blocking solution and shake at room temperature for 0.5~1h. Add 100ul of 5% BSA in Tris solution (0.1M, pH 8.0) and mix vertically at room temperature for 0.5h (blocking). 6、Resuspending: Centrifuge, remove the supernatant, and add an appropriate amount of resuspension solution to resuspend to a quantitative concentration for use. Centrifuge at 10000prm for 10min, remove the supernatant, add 500ul of resuspension solution to dilute to 0.4mg/ml, ultrasonicate for dispersion, and store at 4℃;Precautions1.Inappropriate use of buffer solution may lead to aggregation of markers. It is recommended to add ultrasonic operation during the marking process to disperse the microspheres. 2.Centrifugation conditions should be adjusted accordingly with changes in centrifuge volume. 3.Fluorescent microspheres need to be ultrasonically dispersed before use. 4.Coupling efficiency is affected by pH, temperature, molecular weight, and salt concentration. It is recommended to optimize the reaction conditions. 5. Waste should be disposed of as hazardous waste.

Storage Temperature: Store at 2-8°C;Protected from light

Shipped in: Wet ice

UPC:

41106513

Weight:

1.09 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

EA

WeightUOM:

LB

MPN:

T1521568-1ml

Country of Origin:

China

Product Size:

1ml

Harmonized Tariff Code:

2931.90.90