Tissue Digestion Enzyme (General Purpose) (C008B-268073)

This product contains proteases such as collagenase commonly used in enzymatic tissue digestion. It can specifically degrade extracellular collagen, dissociate the network structure of collagen and fibronectin without damaging the integrity of cell surface structures. Tissue digestion with this product loosens intercellular bridging structures, transforming massive tissues into flocculent substances. After mechanical pipetting, cell suspensions containing small cell clusters and a large number of single cells can be obtained. The prepared cell suspensions are applicable to primary cell culture and cytological researches by flow cytometry, including apoptosis, proliferation, cell cycle and other assays.Application ScopeIt is applicable to various normal and tumor tissues for isolation of primary cells, including epithelial cells, stromal cells, fibroblasts and other cell types.Instructions for Use1. Take the digestive enzyme out of the -20℃ refrigerator in advance and thaw it at 2–8℃.2. Dilute the tissue digestive enzyme at a ratio of 1:1 with digestive enzyme diluent (Catalog No. D152114) to prepare working concentration solution.3. Transfer tissues into a petri dish, add 3 mL tissue washing solution (Catalog No. O1521152) to rinse tissues, and repeat the washing process 2–3 times.4. Under aseptic conditions, mechanically cut tissues into 1–2 mm³ fragments, then transfer them into sterile 15 mL centrifuge tubes.5. Add 5 mL tissue washing solution, and centrifuge at 1500 rpm for 3 minutes at room temperature.6. Discard the supernatant, add 12 mL working-concentration digestive enzyme. Label sample names and numbers, seal the tubes, and place them in a constant-temperature shaking incubator for digestion. Set parameters at 37℃, 200–300 rpm (rotation speed adjusted according to tissue texture). Terminate digestion when tissues become flocculent with no visible hard lumps.7.Take out digested samples and centrifuge at 1500 rpm for 3 minutes at room temperature.8. Discard the supernatant, add 6 mL serum-containing medium to terminate digestion, and centrifuge at 1500 rpm for 3 minutes at room temperature.9. Discard the supernatant and add diluent for subsequent use.Precautions1. The product shall be thawed at 2–8℃ before use and consumed within one week after thawing.2. Strict aseptic operation is required during use to avoid contamination.3. The storage concentration of this product is twice the working concentration, and it must be diluted 1:1 with special diluent before application.4. To maintain optimal performance, the product is generally used at 37℃. It can also be applied at 2–8℃ depending on specific experimental requirements.5. Use the product within the shelf life. Expected experimental effects cannot be guaranteed beyond the expiration date.6. This product is for scientific research only and shall not be used for in vitro diagnosis.

Specifications & Purity: This product contains proteases such as collagenase commonly used in enzymatic tissue digestion. It can specifically degrade extracellular collagen, dissociate the network structure of collagen and fibronectin without damaging the integrity of cell surface structures. Tissue digestion with this product loosens intercellular bridging structures, transforming massive tissues into flocculent substances. After mechanical pipetting, cell suspensions containing small cell clusters and a large number of single cells can be obtained. The prepared cell suspensions are applicable to primary cell culture and cytological researches by flow cytometry, including apoptosis, proliferation, cell cycle and other assays.Application ScopeIt is applicable to various normal and tumor tissues for isolation of primary cells, including epithelial cells, stromal cells, fibroblasts and other cell types.Instructions for Use1. Take the digestive enzyme out of the -20℃ refrigerator in advance and thaw it at 2–8℃.2. Dilute the tissue digestive enzyme at a ratio of 1:1 with digestive enzyme diluent (Catalog No. D152114) to prepare working concentration solution.3. Transfer tissues into a petri dish, add 3 mL tissue washing solution (Catalog No. O1521152) to rinse tissues, and repeat the washing process 2–3 times.4. Under aseptic conditions, mechanically cut tissues into 1–2 mm³ fragments, then transfer them into sterile 15 mL centrifuge tubes.5. Add 5 mL tissue washing solution, and centrifuge at 1500 rpm for 3 minutes at room temperature.6. Discard the supernatant, add 12 mL working-concentration digestive enzyme. Label sample names and numbers, seal the tubes, and place them in a constant-temperature shaking incubator for digestion. Set parameters at 37℃, 200–300 rpm (rotation speed adjusted according to tissue texture). Terminate digestion when tissues become flocculent with no visible hard lumps.7.Take out digested samples and centrifuge at 1500 rpm for 3 minutes at room temperature.8. Discard the supernatant, add 6 mL serum-containing medium to terminate digestion, and centrifuge at 1500 rpm for 3 minutes at room temperature.9. Discard the supernatant and add diluent for subsequent use.Precautions1. The product shall be thawed at 2–8℃ before use and consumed within one week after thawing.2. Strict aseptic operation is required during use to avoid contamination.3. The storage concentration of this product is twice the working concentration, and it must be diluted 1:1 with special diluent before application.4. To maintain optimal performance, the product is generally used at 37℃. It can also be applied at 2–8℃ depending on specific experimental requirements.5. Use the product within the shelf life. Expected experimental effects cannot be guaranteed beyond the expiration date.6. This product is for scientific research only and shall not be used for in vitro diagnosis.

Grade: BioReagent;for Cell culture;Sterile

Storage Temperature: Store at -20°C

Shipped in: Ice chest + Ice pads

UPC:

51172415

Weight:

9.87 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

EA

WeightUOM:

LB

MPN:

O1521148-100ml

Country of Origin:

China

Product Size:

100ml

Harmonized Tariff Code:

2931.90.90