Transhydrogenase-1 Activity Assay Kit (UV Micro Method)

TH is located on the inner mitochondrial membrane and is also known as respiratory electron transport chain complex VI. It catalyzes the interconversion between NADH + NADP⁺ and NAD⁺ + NADPH. The forward reaction is referred to as TH‑1. An increase in mitochondrial NADH content leads to an elevated H⁺ electrochemical gradient across the mitochondrial membrane, thereby promoting ROS production in the electron transport chain. TH‑1 promotes the conversion of NADH to NADPH, thus enhancing the antioxidant capacity of mitochondria. Assay principle: Both NADH and NADPH exhibit characteristic absorption at 340 nm; therefore, the transhydrogenation reaction catalyzed by TH does not cause a change in absorbance at 340 nm. Using the artificial substrate 3-acetylpyridine adenine dinucleotide phosphate (APADP⁺) as a substitute for NADP⁺, TH‑1 catalyzes the reduction of APADP⁺ to APADPH, which has a characteristic absorption peak at 375 nm. Thus, TH‑1 activity is calculated by measuring the rate of increase in absorbance at 375 nm.

Specifications & Purity: TH is located on the inner mitochondrial membrane and is also known as respiratory electron transport chain complex VI. It catalyzes the interconversion between NADH + NADP⁺ and NAD⁺ + NADPH. The forward reaction is referred to as TH‑1. An increase in mitochondrial NADH content leads to an elevated H⁺ electrochemical gradient across the mitochondrial membrane, thereby promoting ROS production in the electron transport chain. TH‑1 promotes the conversion of NADH to NADPH, thus enhancing the antioxidant capacity of mitochondria. Assay principle: Both NADH and NADPH exhibit characteristic absorption at 340 nm; therefore, the transhydrogenation reaction catalyzed by TH does not cause a change in absorbance at 340 nm. Using the artificial substrate 3-acetylpyridine adenine dinucleotide phosphate (APADP⁺) as a substitute for NADP⁺, TH‑1 catalyzes the reduction of APADP⁺ to APADPH, which has a characteristic absorption peak at 375 nm. Thus, TH‑1 activity is calculated by measuring the rate of increase in absorbance at 375 nm.

Grade: BioReagent

Storage Temperature: Store at 2-8°C

Shipped in: Wet ice

UPC:

41141618

Weight:

1.09 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

ST

WeightUOM:

LB

MPN:

T1522132-100T

Country of Origin:

China

Product Size:

100T

Harmonized Tariff Code:

2931.90.90