Transhydrogenase-2 Activity Assay Kit (UV Micro Method)

TH is located on the inner mitochondrial membrane and is also known as mitochondrial complex VI. It catalyzes the interconversion between NADH + NADP⁺ and NAD⁺ + NADPH, thereby regulating the balance of mitochondrial NAD(H) and NADP(H). The reverse reaction is referred to as TH‑2, which catalyzes the conversion of NADPH and NAD⁺ to NADP⁺ and NADH. Assay principle: Both NADH and NADPH exhibit characteristic absorption at 340 nm; therefore, the transhydrogenation reaction catalyzed by TH does not cause a change in absorbance at 340 nm. Using the artificial substrate 3-acetylpyridine adenine dinucleotide (APAD⁺) as a substitute for NAD⁺, TH‑2 catalyzes the reduction of APAD⁺ to APADH, which has a characteristic absorption peak at 375 nm. The TH‑2 activity is calculated by measuring the rate of increase in absorbance at 375 nm.

Specifications & Purity: TH is located on the inner mitochondrial membrane and is also known as mitochondrial complex VI. It catalyzes the interconversion between NADH + NADP⁺ and NAD⁺ + NADPH, thereby regulating the balance of mitochondrial NAD(H) and NADP(H). The reverse reaction is referred to as TH‑2, which catalyzes the conversion of NADPH and NAD⁺ to NADP⁺ and NADH. Assay principle: Both NADH and NADPH exhibit characteristic absorption at 340 nm; therefore, the transhydrogenation reaction catalyzed by TH does not cause a change in absorbance at 340 nm. Using the artificial substrate 3-acetylpyridine adenine dinucleotide (APAD⁺) as a substitute for NAD⁺, TH‑2 catalyzes the reduction of APAD⁺ to APADH, which has a characteristic absorption peak at 375 nm. The TH‑2 activity is calculated by measuring the rate of increase in absorbance at 375 nm.

Grade: BioReagent

Storage Temperature: Store at 2-8°C

Shipped in: Wet ice

UPC:

41141618

Weight:

1.09 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

ST

WeightUOM:

LB

MPN:

T1522134-100T

Country of Origin:

China

Product Size:

100T

Harmonized Tariff Code:

2931.90.90