TRIS-BORATE-EDTA BUFFER WORKING SOLUTION MOLECULAR BIOLOGY REAGENT (C005B-286554)

Application

TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids.
Dilution of the TBE stock concentrates to a 1ƒ TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5ƒ or 10ƒ stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Packaging

Supplied in dispenser with a spigot.

Other Notes

89 mM Tris borate, pH approx. 8.3, containing 2 mM EDTA.

Preparation Note

Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134).

Solution prepared with 18 megohm water

UPC:

41105323

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

EA

MPN:

T9525-1GA