Sigma-Aldrich

TRITON(R) X-100 REDUCED (C005B-275464)

Name: TRITON(R) X-100 REDUCED (C005B-275464)
SKU: C005B-275464
Price: 907.48 USD
Availability: InStock

General description Triton ™: X-100 reduced is a versatile nonionic surfactant featuring a hydrophilic polyethylene oxide chain, commonly employed as a laboratory detergent. Its applications span various purposes, including cell lysis to

Description

General description

Triton^{^™}X-100 reduced is a versatile nonionic surfactant featuring a hydrophilic polyethylene oxide chain, commonly employed as a laboratory detergent. Its applications span various purposes, including cell lysis to extract proteins or organelles, permeabilizing the membranes of living cells, and serving as a vital component in lysis buffers. Widely utilized in biochemical and cell biology research, Triton^™ X-100 facilitates the isolation of lipid rafts, enhancing the solubility and dispersibility of substances. With a reduced polyoxyethylene content of approximately 10, this product exhibits excellent wetting properties and emulsification enhancement.

In cell biology, Triton^{^™} X-100 plays a crucial role in solubilizing membrane-bound proteins, preserving their native conformation in solution. Furthermore, Triton^™ X-100 reduced, resulting from the full hydrogenation of the benzene moiety of TX-100 to a cyclohexane derivative, has been associated with potential benefits in enzyme digestion enhancement and effects on the photoisomerization of bacteriorhodopsin. The versatility of Triton^{^™} X-100 in various research applications underscores its significance as a fundamental tool in biochemical and cell biology investigations.

Application

Triton^™ X-100 reduced has been used:

in nuclear magnetic resonance (NMR) sample tube for lysing/rupturing the 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) phospholipid vesicles
in cell permeabilization
as a component in Tris/HCl to determine hydrolysis of the esters for enzyme activity assays