General description
Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.
Application
Triton™ X-100 has been used:
- for permeabilization of cells before techniques like indirect immunofluorescence and flow cytometry
- As a component of lysis buffer used for homogenization of cells
- As a component of blocking buffer in immunofluorescence staining
- For the preparation of cells in double fluorescence staining
Biochem/physiol Actions
Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.
Features and Benefits
- Suitable for Molecular Biology and Biochemical Research
- Free from DNase, RNase, NICKase and protease
- Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications.
- High purity product for research applications
- Tested for the presence of Anionic and Cationic traces
Legal Information
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
- UPC:
- 51111869
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 93443-100ML