Sigma-Aldrich

TRITON X-100 SOLUTION BIOULTRA 100ML

Name: TRITON X-100 SOLUTION BIOULTRA 100ML
SKU: C005B-089217
Price: 312.2 USD
Availability: InStock

Triton ™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as

Description

General description

Triton^™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Application

Triton^{^™} X-100 has been used:

for permeabilization of cells before techniques like indirect immunofluorescence and flow cytometry
As a component of lysis buffer used for homogenization of cells
As a component of blocking buffer in immunofluorescence staining
For the preparation of cells in double fluorescence staining

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Features and Benefits

Suitable for Molecular Biology and Biochemical Research
Free from DNase, RNase, NICKase and protease
Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications.
High purity product for research applications
Tested for the presence of Anionic and Cationic traces