Sigma-Aldrich

SIGMA Trizmar base, Primary Standard and Buffer, =99.9% (tit

Name: SIGMA Trizmar base, Primary Standard and Buffer, =99.9% (tit
SKU: C005B-298941
Price: 194.91 USD
Availability: InStock

General description Tris(hydroxymethyl)aminomethane, commonly known as Trometamol, Tris base, or Trizma ®: base, serves a pivotal role in diverse research applications as a biological buffer. Its optimal pKa of 8.

Description

General description

Tris(hydroxymethyl)aminomethane, commonly known as Trometamol, Tris base, or Trizma^{^®} base, serves a pivotal role in diverse research applications as a biological buffer. Its optimal pKa of 8.1 makes it a preferred choice for formulating buffers like Tris-acetate-EDTA (TAE) and Tris-borate-EDTA (TBE), ensuring the maintenance of pH within the physiological range (pH 7 - 9), applicable to a wide spectrum of living organisms. Despite its utility, researchers need to exercise caution in protein studies, as Tris has the potential to interfere with the activity of specific enzymes.

Tris base may find application as basimetric standard, independently as a buffer and as a crucial component in mixed buffer formulations, including Tris-EDTA (TE) buffer, TAE buffer, TBE buffer, among others. Its attributes include purity, essential stability, and a relative non-hygroscopic nature, making it a dependable choice in laboratory settings. In these environments, Tris base is indispensable for preparing buffers compatible with biological fluids and serves as a standard pH solution. It facilitates various laboratory procedures such as lactate dehydrogenase assays, in situ hybridization, and protein extraction from cells. The versatility of Tris base extends to cell biology, biochemistry, and protein research contributing significantly to studies involving cell membrane permeability and buffer preparation.

Application

Trizma^® base has been used:

as a component of H buffer (cell dissociation buffer)
for washing and saturation of wells in double sandwich ELISA immunoenzymatic technique
as an assay buffer for reconstitution of extracted and dried protein samples
to prepare Tris-HCl buffer that is used to stabilize proteins
as a buffer to extract carotenoid from tubers
as a component of sample buffer during protein extraction prior to western blotting
as a component of sample buffer for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)
to prepare simulated body fluid (SBF) for calcium phosphate (CaP) resorption assay
as a buffer for polydopamine (PDA) deposition on stainless steel (SS) substrate

Features and Benefits

Efficient buffering within the pH range of 7 - 9 with a pKa of 8.1 (25 °C)
Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
Can be used in Cell Biology, and Biochemical research

Other Notes

Easily compare specifications for Trizma products with the Trizma specification table.

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.