Trypsin/Lys-C Mix (MS)

This product contains trypsin and Lys-C protease, which are recombinantly expressed metalloproteases. Trypsin specifically cleaves peptide bonds at the C-terminus of arginine (R) and lysine (K) residues with ultra-fast kinetics; Lys-C protease specifically cleaves peptide bonds at the C-terminus of lysine (K) residues rapidly. Since trypsin cannot digest arginine and lysine at the same rate, many lysine residues are missed. Additionally, post-translational modification (PTM) on lysine or adjacent strongly hydrophobic amino acids (e.g., proline, P) at the C-terminus can also cause missed cleavages. The trypsin/Lys-C protease cocktail is suitable for processing complex protein samples that are difficult to digest. It is applied in protein characterization, single-cell omics, and large-cohort proteomics research. Reconstitution Solution: Reconstitute with 40 μL of 50 mmol/L acetic acid to obtain a concentration of 0.5 μg/μL. pH Range: The protease cocktail exhibits optimal activity at pH 7–9. Digestion Protocol: Recommended Digestion Buffer: 20 mmol/L Tris, 50 mmol/L ABC, or HEPES buffer (pH 7–9). Denatured Protein Sample: Use a protease-to-protein ratio of 1:50 (w/w). Adjust the protein concentration in the digestion reaction to 0.5 μg/μL, and incubate in a dry bath at 37°C for 30 minutes.

Specifications & Purity: This product contains trypsin and Lys-C protease, which are recombinantly expressed metalloproteases. Trypsin specifically cleaves peptide bonds at the C-terminus of arginine (R) and lysine (K) residues with ultra-fast kinetics; Lys-C protease specifically cleaves peptide bonds at the C-terminus of lysine (K) residues rapidly. Since trypsin cannot digest arginine and lysine at the same rate, many lysine residues are missed. Additionally, post-translational modification (PTM) on lysine or adjacent strongly hydrophobic amino acids (e.g., proline, P) at the C-terminus can also cause missed cleavages. The trypsin/Lys-C protease cocktail is suitable for processing complex protein samples that are difficult to digest. It is applied in protein characterization, single-cell omics, and large-cohort proteomics research. Reconstitution Solution: Reconstitute with 40 μL of 50 mmol/L acetic acid to obtain a concentration of 0.5 μg/μL. pH Range: The protease cocktail exhibits optimal activity at pH 7–9. Digestion Protocol: Recommended Digestion Buffer: 20 mmol/L Tris, 50 mmol/L ABC, or HEPES buffer (pH 7–9). Denatured Protein Sample: Use a protease-to-protein ratio of 1:50 (w/w). Adjust the protein concentration in the digestion reaction to 0.5 μg/μL, and incubate in a dry bath at 37°C for 30 minutes.

Grade: Recombinant;Suitable for mass spectrometry (MS);EnzymoPure™

EC Number: 607-831-3

Storage Temperature: Store at -20°C;Avoid repeated freezing and thawing

Shipped in: Ice chest + Ice pads

UPC:

41105803

Weight:

1.09 Ounces

HazmatClass:

Yes

Quantity:

1

Unit of Measurement:

EA

WeightUOM:

LB

MPN:

T1523244-20μg

Country of Origin:

China

CAS:

9002-07-7

Product Size:

20μg

Harmonized Tariff Code:

2931.90.90