Trypsin (MS) (C008B-268674)

This product is a recombinantly expressed metalloprotease. It ultra-fast and specifically cleaves the C-terminal peptide bonds of arginine (R) and lysine (K) residues. 经过 redesigned at the gene sequence level and modified by methylation, this protease features ultra-rapid protein digestion, resistance to autolysis, and high activity. It is intended for use in protein characterization, single-cell omics, and large-cohort proteomics research. Physical Form: Lyophilized powder containing 20 μg of trehalose. Reconstitution Solution: Reconstitute with 20 μL of 1 mmol/L hydrochloric acid to achieve a concentration of 1 μg/μL. pH Range: Optimal activity is achieved at pH 7–9. Digestion Protocol: Digestion Buffer: 20 mmol/L Tris, 50 mmol/L ABC, or HEPES buffer; pH range 7–9. Denatured Protein Samples: Use a protease-to-protein ratio of 1:50 (w/w). Control the protein concentration in the digestion reaction to 0.5 μg/μL, and incubate in a dry bath at 45°C for 30 minutes.

Specifications & Purity: This product is a recombinantly expressed metalloprotease. It ultra-fast and specifically cleaves the C-terminal peptide bonds of arginine (R) and lysine (K) residues. 经过 redesigned at the gene sequence level and modified by methylation, this protease features ultra-rapid protein digestion, resistance to autolysis, and high activity. It is intended for use in protein characterization, single-cell omics, and large-cohort proteomics research. Physical Form: Lyophilized powder containing 20 μg of trehalose. Reconstitution Solution: Reconstitute with 20 μL of 1 mmol/L hydrochloric acid to achieve a concentration of 1 μg/μL. pH Range: Optimal activity is achieved at pH 7–9. Digestion Protocol: Digestion Buffer: 20 mmol/L Tris, 50 mmol/L ABC, or HEPES buffer; pH range 7–9. Denatured Protein Samples: Use a protease-to-protein ratio of 1:50 (w/w). Control the protein concentration in the digestion reaction to 0.5 μg/μL, and incubate in a dry bath at 45°C for 30 minutes.

Grade: Recombinant;Suitable for mass spectrometry (MS);EnzymoPure™

EC Number: 607-831-3

Storage Temperature: Store at -20°C;Avoid repeated freezing and thawing

Shipped in: Ice chest + Ice pads

UPC:

51283224

Weight:

1.09 Ounces

HazmatClass:

Yes

Quantity:

1

Unit of Measurement:

EA

WeightUOM:

LB

MPN:

T1523236-20μg

Country of Origin:

China

CAS:

9002-07-7

Product Size:

20μg

Harmonized Tariff Code:

2931.90.90