Trypsin (MS) (C008B-268675)

This product is a recombinantly expressed metalloprotease. It specifically cleaves peptide bonds at the C-terminus of arginine (R) and lysine (K) residues with ultra-fast kinetics. Derived from Fusarium species, this protease features ultra-rapid protein digestion, autolysis resistance, and high activity. It is used in protein characterization, single-cell omics, and adherent cell dissociation studies. Reconstitution Solution: Reconstitute with 40 μL of ultrapure water or DPBS for cell dissociation applications. pH Range: rTrypsin 5.0 exhibits optimal activity at pH 7–9. Digestion Protocol: Recommended Digestion Buffer: 20 mmol/L PBS, 50 mmol/L ABC, or HEPES buffer (pH 7–9). Denatured Protein Sample: Use a protease-to-protein ratio of 1:50 (w/w). Adjust the protein concentration in the digestion reaction to 0.5 μg/μL, and incubate in a dry bath at 37°C for 30 minutes.

Specifications & Purity: This product is a recombinantly expressed metalloprotease. It specifically cleaves peptide bonds at the C-terminus of arginine (R) and lysine (K) residues with ultra-fast kinetics. Derived from Fusarium species, this protease features ultra-rapid protein digestion, autolysis resistance, and high activity. It is used in protein characterization, single-cell omics, and adherent cell dissociation studies. Reconstitution Solution: Reconstitute with 40 μL of ultrapure water or DPBS for cell dissociation applications. pH Range: rTrypsin 5.0 exhibits optimal activity at pH 7–9. Digestion Protocol: Recommended Digestion Buffer: 20 mmol/L PBS, 50 mmol/L ABC, or HEPES buffer (pH 7–9). Denatured Protein Sample: Use a protease-to-protein ratio of 1:50 (w/w). Adjust the protein concentration in the digestion reaction to 0.5 μg/μL, and incubate in a dry bath at 37°C for 30 minutes.

Grade: Recombinant;Suitable for mass spectrometry (MS);EnzymoPure™

EC Number: 607-831-3

Storage Temperature: Store at 2-8°C

Shipped in: Wet ice

UPC:

51283224

Weight:

1.09 Ounces

HazmatClass:

Yes

Quantity:

1

Unit of Measurement:

EA

WeightUOM:

LB

MPN:

T1523240-20μg

Country of Origin:

China

CAS:

9002-07-7

Product Size:

20μg

Harmonized Tariff Code:

2931.90.90