XANTHINE OXIDASE FROM MICROORGANISM (C005B-275440)

General description

Research area: Cell Cycle

Xanthine Oxidase (XO), a versatile molybdoflavoprotein, is one of the forms of xanthine oxidoreductases (XORs). XO is characterized with two flavin molecules (FAD), two molybdenum atoms, and eight iron atoms bound per enzymatic unit.

Application

Xanthine Oxidase microbial has been used:

• to determine in vitro xanthine oxidase activity
• as a source to generate superoxide anions during superoxide dismutase assay
• as a component of the reagent mix in colorimetric paper-based device to detect allopurinol in traditional medicine by enzymatic reaction

Biochem/physiol Actions

Xanthine Oxidase catalyzes the hydroxylation of hypoxanthine to xanthine and xanthine to uric acid and oxygen free radicals during purine metabolism. This enzyme is also implicated in caffeine metabolism. XO also plays a vital role in the catabolism of xenobiotics including various drugs such as thiopurine drugs, containing 6-mercaptopurine, allopurinol, and uric acid. Excess of XO activity causes hyperuricemia (higher levels of uric acid in the blood), which further leads to gout.

Also contains proteolytic activity.

Physical properties

Inhibitors: Reducing agents, Hg⁺⁺, Ag⁺,MIA
Optimum pH: 7.5 - 8.0
Optimum temperature: 65^oC
pH Stability: pH 6.5 - 9.0 (25^oC, 15hr)
Thermal stability: below 55^oC (pH 8.0, 30min)

Unit Definition

One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.

Physical form

Lyophilized powder containing BSA and sodium glutamate as stabilizers

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UPC:

41115406

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

100

Unit of Measurement:

UN

MPN:

X2252-100UN

CAS:

9002-17-9

Temperature Control Device:

Yes