Zymolyase, Arthrobacter luteus

Zymolyase, produced by a submerged culture of Arthrobacter luteus, has strong lytic activity against living yeast cell walls, to produce protoplast or spheroplast of various strains of yeast cells. An essential enzyme for the lytic activity of Zymolyase is β-1,3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with β-1,3-linkages and releases specifically laminaripentaose as the main and minimum product unit.Product InformationFormLyophilized PowderPurificationAmmonium Sulfate PrecipitationActivity20,000 units/gEssential enzymeβ-1,3-glucan laminaripentaohydrolaseOther activitiescontained (※1)β-1,3-glucanase:  approx. 1.5 × 10⁶ units/gProtease: approx. 1.0 × 10⁴ units/gMannanase: approx. 1.0 × 10⁶ units/gContaminants (Amylase, Xylanase, Phosphatase)Trace amountOptimum pH and Temp.pH 7.5, 35°C (for lysis of viable yeast cells)pH 6.5, 45°C (for hydrolysis of yeast glucan)Stability (2°C)No loss of activity was found after storage for 1 yearHeat stability30°C: 70% of the lytic activity is lost after storage for 3 months90% of the lytic activity is lost after storage for 3 months60°C:  Lytic activity is lost on incubation for 5 minutesSpecificity (Lytic Spectrum)Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, Kluyveromyces, Lipomyces, Metschnikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, Schwanniomyces, etc.(※1) See reference, Kitamura, K., Kaneko, T., Yamamoto, Y., J. Gen. Appl. Microbiol., 18, 57 (1972) as to the definition of each enzyme units.Unit DefinitionOne unit of lytic activity is defined as that amount which indicates 30% of decrease in absorbance at 800 nm(A800) of the reaction mixture under the following condition.Reaction MixtureEnzyme solution1 ml (0.05–0.1 mg/ml for Zymolyase)Brewer's yeast cell suspension3 ml (2 mg/ml)1/15M Phosphate buffer5 ml (pH7.5)Distilled water1 mlAfter incubation for 2 hours at 25°C with gentle shaking, A800 of the mixture is determined. When 60% of A800 decrease, equivalent to 2 units, is observed in the reaction system, the brewer’s yeast cells are completely lysed, namely 1 unit of Zymolyase lyses 3 mg dry weight of brewer’s yeast. Preparation of 1/15 M Phosphate Buffer (1L of pH 7.5 buffer)Dissolve 19.1 g of di-sodium hydrogenphosphate 12-hydrate in distilled water to make an 800 mL solution.Dissolve 1.814 g of potassium dihydrogenphosphate in distilled water to make a 200 mL solution.Mix the two solutions.The below phosphate buffers (pH 7.5) have been tested for compatibility.   

Specifications & Purity: Zymolyase, produced by a submerged culture of Arthrobacter luteus, has strong lytic activity against living yeast cell walls, to produce protoplast or spheroplast of various strains of yeast cells. An essential enzyme for the lytic activity of Zymolyase is β-1,3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with β-1,3-linkages and releases specifically laminaripentaose as the main and minimum product unit.Product InformationFormLyophilized PowderPurificationAmmonium Sulfate PrecipitationActivity20,000 units/gEssential enzymeβ-1,3-glucan laminaripentaohydrolaseOther activitiescontained (※1)β-1,3-glucanase:  approx. 1.5 × 10⁶ units/gProtease: approx. 1.0 × 10⁴ units/gMannanase: approx. 1.0 × 10⁶ units/gContaminants (Amylase, Xylanase, Phosphatase)Trace amountOptimum pH and Temp.pH 7.5, 35°C (for lysis of viable yeast cells)pH 6.5, 45°C (for hydrolysis of yeast glucan)Stability (2°C)No loss of activity was found after storage for 1 yearHeat stability30°C: 70% of the lytic activity is lost after storage for 3 months90% of the lytic activity is lost after storage for 3 months60°C:  Lytic activity is lost on incubation for 5 minutesSpecificity (Lytic Spectrum)Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, Kluyveromyces, Lipomyces, Metschnikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, Schwanniomyces, etc.(※1) See reference, Kitamura, K., Kaneko, T., Yamamoto, Y., J. Gen. Appl. Microbiol., 18, 57 (1972) as to the definition of each enzyme units.Unit DefinitionOne unit of lytic activity is defined as that amount which indicates 30% of decrease in absorbance at 800 nm(A800) of the reaction mixture under the following condition.Reaction MixtureEnzyme solution1 ml (0.05–0.1 mg/ml for Zymolyase)Brewer's yeast cell suspension3 ml (2 mg/ml)1/15M Phosphate buffer5 ml (pH7.5)Distilled water1 mlAfter incubation for 2 hours at 25°C with gentle shaking, A800 of the mixture is determined. When 60% of A800 decrease, equivalent to 2 units, is observed in the reaction system, the brewer’s yeast cells are completely lysed, namely 1 unit of Zymolyase lyses 3 mg dry weight of brewer’s yeast. Preparation of 1/15 M Phosphate Buffer (1L of pH 7.5 buffer)Dissolve 19.1 g of di-sodium hydrogenphosphate 12-hydrate in distilled water to make an 800 mL solution.Dissolve 1.814 g of potassium dihydrogenphosphate in distilled water to make a 200 mL solution.Mix the two solutions.The below phosphate buffers (pH 7.5) have been tested for compatibility.   

Grade: Bioactive;ActiBioPure™;Native;High Performance;EnzymoPure™

EC Number: 609-409-4

Storage Temperature: Store at 2-8°C

Shipped in: Wet ice

UPC:

51451804

Weight:

1.09 Ounces

HazmatClass:

Yes

Quantity:

1

Unit of Measurement:

EA

WeightUOM:

LB

MPN:

Z1421592-1g

Country of Origin:

China

CAS:

37340-57-1

Product Size:

1g

Harmonized Tariff Code:

2931.90.90