Zymolyase (C008B-268833)

Zymolyase, produced by a submerged culture of Arthrobacter luteus, has strong lytic activity against living yeast cell walls, to produce protoplast or spheroplast of various strains of yeast cells. An essential enzyme for the lytic activity of Zymolyase is β-1,3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with β-1,3-linkages and releases specifically laminaripentaose as the main and minimum product unit. Essential enzyme: β-1,3-glucan laminaripentaohydrolase ptimum pH and Temp.: pH7.5, 35°C (for lysis of viable yeast cells) pH6.5, 45°C (for hydrolysis of yeast glucan) Stability (2°C): No loss of activity was found after storage for 1 year Specificity (Lytic Spectrum) : Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, kluyveromyces, Lipomyces, Metschnikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, Schwanniomyces, etc. Unit Definition One unit of lytic activity is defined as that amount which indicates 30% of decrease in absorbance at 800 nm(A800) of the reaction mixture under the following condition. Reaction Mixture Enzyme solution: 1 ml (0.012-0.024 mg/ml for Zymolyase) Brewer's yeast cell suspension: 3 ml (2 mg/ml) 1/15M Phosphate buffer: 5 ml (pH7.5) Distilled water: 1 ml After incubation for 2 hours at 25°C with gentle shaking, A800 of the mixture is determined. When 60% of A800 decrease, equivalent to 2 units, is observed in the reaction system, the brewer’s yeast cells are completely lysed, namely 1 unit of Zymolyase lyses 3 mg dry weight of brewer’s yeast. Preparation of 1/15 M Phosphate Buffer (1L of pH 7.5 buffer) Dissolve 19.1 g of di-sodium hydrogenphosphate 12-hydrate in distilled water to make an 800 mL solution. Dissolve 1.814 g of potassium dihydrogenphosphate in distilled water to make a 200 mL solution. Mix the two solutions.

Specifications & Purity: Zymolyase, produced by a submerged culture of Arthrobacter luteus, has strong lytic activity against living yeast cell walls, to produce protoplast or spheroplast of various strains of yeast cells. An essential enzyme for the lytic activity of Zymolyase is β-1,3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with β-1,3-linkages and releases specifically laminaripentaose as the main and minimum product unit. Essential enzyme: β-1,3-glucan laminaripentaohydrolase ptimum pH and Temp.: pH7.5, 35°C (for lysis of viable yeast cells) pH6.5, 45°C (for hydrolysis of yeast glucan) Stability (2°C): No loss of activity was found after storage for 1 year Specificity (Lytic Spectrum) : Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, kluyveromyces, Lipomyces, Metschnikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, Schwanniomyces, etc. Unit Definition One unit of lytic activity is defined as that amount which indicates 30% of decrease in absorbance at 800 nm(A800) of the reaction mixture under the following condition. Reaction Mixture Enzyme solution: 1 ml (0.012-0.024 mg/ml for Zymolyase) Brewer's yeast cell suspension: 3 ml (2 mg/ml) 1/15M Phosphate buffer: 5 ml (pH7.5) Distilled water: 1 ml After incubation for 2 hours at 25°C with gentle shaking, A800 of the mixture is determined. When 60% of A800 decrease, equivalent to 2 units, is observed in the reaction system, the brewer’s yeast cells are completely lysed, namely 1 unit of Zymolyase lyses 3 mg dry weight of brewer’s yeast. Preparation of 1/15 M Phosphate Buffer (1L of pH 7.5 buffer) Dissolve 19.1 g of di-sodium hydrogenphosphate 12-hydrate in distilled water to make an 800 mL solution. Dissolve 1.814 g of potassium dihydrogenphosphate in distilled water to make a 200 mL solution. Mix the two solutions.

Grade: Bioactive;ActiBioPure™;Native;High Performance;EnzymoPure™

EC Number: 609-409-4

Storage Temperature: Store at 2-8°C

Shipped in: Wet ice

UPC:

51302327

Weight:

1.09 Ounces

HazmatClass:

Yes

Quantity:

1

Unit of Measurement:

EA

WeightUOM:

LB

MPN:

Z1523272-100mg

Country of Origin:

China

CAS:

37340-57-1

Product Size:

100mg

Harmonized Tariff Code:

2931.90.90