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a2 hamster chinese ovary (c15-1311-379)

A2 HAMSTER CHINESE OVARY (C15-1311-379)

Catalog No.
C15-1311-379
Manufacturer No.
89030316-1VL
Manufacturer Name
Sigma-Aldrich
Quantity
1
Unit of Measure
VI
Price: $1,630.29
List Price: $1,811.43

Cell Line Origin Hamster Chinese ovary Cell Line Description Overproduces ornithine decarboxylase in response to polyamine deprivation. Application Enzymatic studies Culture Medium EMEM (EBSS) + 2mM Glutamine + Ham′s F12 (without Putrescine)

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Cell Line Origin

Hamster Chinese ovary

Cell Line Description

Overproduces ornithine decarboxylase in response to polyamine deprivation.

Application

Enzymatic studies

Culture Medium

EMEM (EBSS) + 2mM Glutamine + Ham′s F12 (without Putrescine) (1:1) + 0.1% BSA.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000 to 1x10,000 cells/cm2 using cold PBS and 0.25% cold trypsin. Place flasks at 4°C for 5-10 minutes or until cells detach. Culture cells at 37°C; 5% CO2. Pre-soak flasks in porcine

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source: hamster ovary. growth mode: Adherent. karyotype: Not specified. morphology: Epithelial. products: Ornithine decarboxylase. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.
UPC:
41181947
Condition:
New
Weight:
1.00 Ounces
HazmatClass:
No
WeightUOM:
LB
MPN:
89030316-1VL


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