Cell Line Origin
Rat Y3Ag1.2.3 x Rat F344 spleen
Cell Line Description
The antibody specific for NADH nitrate reductase and inhibits the reduction of nitrate to nitrite using NADH as electron donor. It also inhibits the reduction of nitrate using flavin nucleotides (FMNH2 or FADH2) and methyl viologen. The antibody is directed against NADH-NR of mono and dicotyledons.
Immunogen
Nitrate reductase purified from the leaves of 6 to 8 week old spinach plants - Spinacea oleracea l
Culture Medium
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation
Other Notes
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
biological source: Rat x Rat Hybridoma. ImmunoDon: F344 rat spleen. Myeloma: Y3Ag1.2.3. Reactivity: Anti-Nitrate Reductase. technique(s): cell culture | mammalian: suitable. isotype: IgG2a. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41116113
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 85102261-1VL
