ANTI-FASN MOUSE MAB (3F2-1F3 1PC X 50UG

General description

Anti-FASN, mouse monoclonal, clone 3F2-1F3, recognizes FASN protein in 293 and HeLa cells and in human breast carcinoma tissue. It is validated for ELISA, WB, ICC, and IHC (paraffin sections).

Purified mouse monoclonal antibody. Recognizes the FASN protein.

Recognizes the FASN protein in 293 and HeLa cells and human breast carcinoma tissue.

Immunogen

Full-length, recombinant human FASN (aa 1-440) expressed as GST fusion protein

Application

ELISA (see coments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Paraffin Sections (10 g/ml)

Warning

Toxicity: Regulatory Review (Z)

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
293 cells, HeLa cells, Human breast carcinoma tissue

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Kristiansen, G., et al. 2010. Br. J. Cancer102, 1736.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Shipping Information:

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UPC:

41181824

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

EA

MPN:

ST1549-50UG

Temperature Control Device:

Yes