General description
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-bromo4-chloro-3-indolyl phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end-product that is blue to purple and can be observed visually. BCIP®/NBT-blue liquid substrate system for membranes is a specifically formulated liquid enzyme substrate that is stable and optimized for blotting applications offers maximum convenience, time savings, and consistency.
Application
BCIP®/NBT-Blue Liquid Substrate System for Membranes has been used in colorimetric detection of alkaline phosphatase (ALP) activity such as western blot analysis.
Caution
Stable at least one year at 2-8 °C. This substrate is light-sensitive.
Other Notes
Not recommended for microwell (e.g., ELISA) or immunohistochemical applications.
Legal Information
BCIP is a registered trademark of Sigma-Aldrich Co. LLC
- UPC:
- 41105106
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- B3804-100ML