Cell Line Origin
Hamster Chinese ovary
Cell Line Description
A subclone of the parental CHO cell line originated by Puck in 1957. The cells have an absolute requirement for L-proline.
Application
CHO Cell Line from Chinese hamster ovary has been used:
- to generate CHO cells expressing LY6/PLAUR domain containing 3 (LYPD3)
- to carry out the vaginolysin (VLY) cytolytic activity assay
- as host cells to produce glucagon-like peptide 1 receptor (GLP1R)
Nutritional and gene expression studies
Culture Medium
Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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biological source: hamster ovary. availability: not available in Japan. growth mode: Adherent. karyotype: Hypodiploid, modal no. 20. morphology: Epithelial. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41106514
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 85050302-1VL