Cell Line Origin
Human lymphoblastoid, normal,EBV-transformed
Cell Line Description
Derived from mononuclear cells from peripheral blood of a 49 year old normal male. Cells were used as controls for cellular radiosensitivity studies and analysis of cell survival after UV-irradiation. The line LB173 has been established by subsequent transformation with EBV. Fibroblasts from the same individual have been designated 144BR.
Application
Mutation studies
Culture Medium
RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).
Subculture Routine
Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO2, 37°C. Cells form floating aggregates.
Other Notes
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biological source: human blood. growth mode: Suspension. karyotype: Not specified. morphology: Lymphoblastoid. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41106514
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 98062316-1VL
